abstract：:The multisolute osmotic virial equation is the only multisolute thermodynamic solution theory that has been derived from first principles and can make predictions of multisolute solution behaviour in the absence of multisolute solution data. Other solution theories either (i) include simplifying assumptions that do no...

journal_title：Cryobiology

authors： Prickett RC,Elliott JA,McGann LE

更新日期：2010-02-01 00:00:00

abstract：:This brief paper introduces the subject of thermodynamics and the papers of the special issue on thermodynamic aspects of cryobiology. Thermodynamic terminology is defined for the non-specialist. ...

journal_title：Cryobiology

authors： Elliott JA

更新日期：2010-02-01 00:00:00

abstract：:In this study, we evaluated various parameters of sperm cryopreservation in two livebearers, guppies (Poecilia reticulata) and black mollies (P. latipinna). Our results suggested a common freezing protocol for the guppies and mollies: suspend sperm in Hanks' balanced salt solution (HBSS) at 300 mOsm/kg, use 14% glycer...

journal_title：Cryobiology

authors： Huang C,Sun C,Su X,Zhao X,Miao M,Liu Y,Dong Q

更新日期：2009-12-01 00:00:00

abstract：:Natural freeze tolerance depends on numerous biochemical adaptations that address the multiple stresses imposed on cells by freezing and preserves viability by suppressing energy-expensive cell functions in the frozen state. We hypothesized that microRNAs, small non-coding RNA transcripts that bind to mRNA, could act ...

journal_title：Cryobiology

authors： Biggar KK,Dubuc A,Storey K

更新日期：2009-12-01 00:00:00

abstract：:The Publisher regrets that this article is an accidental duplication of an article that has already been published, doi:10.1016/j.cryobiol.2009.05.007. The duplicate article has therefore been withdrawn. ...

journal_title：Cryobiology

authors： Wowk B

更新日期：2009-06-06 00:00:00

abstract：:Desiccation preservation holds promise as a simplified alternative to cryopreservation for the long term storage of cells. We report a study on the protective effects of intracellular and extracellular sugars during bovine sperm desiccation and the supplemental effects of the addition of an antioxidant (catalase) or a...

journal_title：Cryobiology

authors： Sitaula R,Elmoazzen H,Toner M,Bhowmick S

更新日期：2009-06-01 00:00:00

abstract：:It is known that life processes below the melting point temperature can actively evolve and establish in micrometer-sized (and larger) veins and structures in ice and permafrost soil, filled with unfrozen water. Thermodynamic arguments and experimental results indicate the existence of much smaller nanometer sized thi...

journal_title：Cryobiology

authors： Möhlmann DT

更新日期：2009-06-01 00:00:00

abstract：:One of the recent approaches to enhance desiccation tolerance in red blood cells (RBCs) is by loading trehalose. This process has been shown to increase the recovery of lyophilized RBCs; conversely, it results in cellular damage including hemoglobin oxidation and loss of membrane integrity. The purpose of this study w...

journal_title：Cryobiology

authors： Kanias T,Acker JP

更新日期：2009-04-01 00:00:00

abstract：:The purpose of this study was to investigate the long-term stabilization of the liver S9-fraction that is widely used in genotoxicity assays in order to mimic bio-activating processes of xenobiotics in vitro. A successful long-term stabilization of the S9-fraction meets the growing demand for the construction of a lab...

journal_title：Cryobiology

authors： Buchinger S,Campen E,Helmers E,Morosow V,Krefft M,Reifferscheid G

更新日期：2009-04-01 00:00:00

abstract：:Vitrification is a promising alternative to tissue preservation, in which the tissue is permeated with cryoprotective agents (CPAs) in order to circumvent the hazardous effects associated with ice formation. In this study, we evaluate the effect of vitreous cryopreservation of rabbit trachea, by comparing vitrificatio...

journal_title：Cryobiology

authors： Xu H,Shi HC,Zang WF,Lu D

更新日期：2009-04-01 00:00:00

abstract：:A high-speed video cryomicroscopy system was developed, and used to observe the process of intracellular ice formation (IIF) during rapid freezing (130 degrees C/min) of bovine pulmonary artery endothelial cells adherent to glass substrates, or in suspension. Adherent cells were micropatterned, constraining cell attac...

journal_title：Cryobiology

authors： Stott SL,Karlsson JOM

更新日期：2009-02-01 00:00:00

abstract：:The freeze tolerance and accumulation of cryoprotectants was investigated in three geographically different populations of the enchytraeid Enchytraeus albidus (Oligochaeta). E. albidus is widely distributed from the high Arctic to temperate Western Europe. Our results show that E. albidus is freeze tolerant, with free...

journal_title：Cryobiology

authors： Slotsbo S,Maraldo K,Malmendal A,Nielsen NC,Holmstrup M

更新日期：2008-12-01 00:00:00

abstract：:Alternative techniques for the cryopreservation of kangaroo spermatozoa that reduced or eliminated the need for glycerol were investigated including; (1) freezing spermatozoa with 20% glycerol in pre-packaged 0.25 mL Cassou straws to enable rapid dilution of the glycerol post-thaw, (2) investigating the efficacy of 20...

journal_title：Cryobiology

authors： McClean R,Zee YP,Holt WV,Johnston SD

更新日期：2008-12-01 00:00:00

abstract：:A lipoprotein-like antifreeze protein (type IV AFP) has previously been isolated only from the blood plasma of the longhorn sculpin. However, the plasma antifreeze activity in all individuals of this species tested from Newfoundland and New Brunswick waters ranges from low to undetectable. A close relative of the long...

journal_title：Cryobiology

authors： Gauthier SY,Scotter AJ,Lin FH,Baardsnes J,Fletcher GL,Davies PL

更新日期：2008-12-01 00:00:00

abstract：:The performance of a small-scale automated cryopreservation and storage system (Mini-BioArchive system) used in the banking of umbilical cord blood (UCB) units was evaluated. After thawing the units, the viability and recovery of cells, as well as the recovery rate of hematopoietic progenitor cells (HPCs) such as CD34...

journal_title：Cryobiology

authors： Miura J,Minegishi M,Itoh T,Kitaura T,Fukawa N,Takahashi H,Suzuki A,Kudo Y,Narita A,Sato Y,Suzuki M,Wada Y,Takeyama Y,Watanabe T,Tsuchiya S

更新日期：2008-10-01 00:00:00

abstract：:Attempts were made to develop a simplified procedure for long-term cryopreservation of intestinal smooth muscle cells (ISMC). ISMC were collected from the ileum of Sprague-Dawley neonatal rats through cellular dissociation in trypsin. Cryopreservation method comprised of a rapid 1-step (protocol 1) and a slow 3-step (...

journal_title：Cryobiology

authors： Batista Lobo S,Denyer M,Gopalan RC,Javid FA

更新日期：2008-10-01 00:00:00

abstract：:One of the major challenges in cryosurgery is to minimize incomplete cryodestruction near the edge of the iceball. In the present study, the feasibility and effectiveness of an amino acidic adjuvant, glycine was investigated to enhance the cryodestruction of MCF-7 human breast cancer cell at mild freezing/thawing cond...

journal_title：Cryobiology

authors： Wang CL,Teo KY,Han B

更新日期：2008-08-01 00:00:00

abstract：:The technical establishment of boar sperm cryopreservation is indispensable for effective breeding of the scarce Okinawan native pig Agu. The objective of the present study was to determine whether ascorbic acid 2-O-alpha-glucoside (AA-2G), a stable ascorbate derivative, is capable of improving the quality of cryopres...

journal_title：Cryobiology

authors： Yoshimoto T,Nakamura S,Yamauchi S,Muto N,Nakada T,Ashizawa K,Tatemoto H

更新日期：2008-08-01 00:00:00

abstract：:Our objective was to study the effect of the concentration of ethylene glycol (EG) and dimethyl sulfoxide (Me2SO) during vitrification on the development of porcine blastocysts. Vitrification was performed with 0.4 M sucrose and either a Me2SO and EG mixture (15%, 16% and 17% v/v of each) or EG alone (40% v/v), using ...

journal_title：Cryobiology

authors： Cuello C,Sanchez-Osorio J,Almiñana C,Gil MA,Perals ML,Lucas X,Roca J,Vazquez JM,Martinez EA

更新日期：2008-06-01 00:00:00

abstract：:This study investigated enzymatic activity of cathepsins and the membrane integrity of zebrafish (Danio rerio) oocytes after freezing to -196 degrees C using controlled slow cooling. Stage III oocytes (>0.5mm), obtained through dissection of anaesthetised female fish and desegregation of ovarian cumulus, were exposed ...

journal_title：Cryobiology

authors： Zhang T,Rawson DM,Tosti L,Carnevali O

更新日期：2008-04-01 00:00:00

abstract：:We have previously shown that cold-induced injury to hepatocytes and liver endothelial cells occurs predominantly via an iron-dependent pathway. However, other groups have reported evidences suggesting that Ca(2+) ions could be involved in the process of cold-induced injury of liver cells. We here assessed the relativ...

journal_title：Cryobiology

authors： Knoop S,de Groot H,Rauen U

更新日期：2008-04-01 00:00:00

abstract：:Organ preservation solutions have been designed to protect grafts against the injury inflicted by cold ischemia. However, toxicity of University of Wisconsin (UW) solution during rewarming has been reported. Therefore, we here assessed the toxicity of UW, histidine-tryptophan-ketoglutarate (HTK), Euro-Collins, histidi...

journal_title：Cryobiology

authors： Rauen U,de Groot H

更新日期：2008-02-01 00:00:00

abstract：:Scientific studies on cryopreservation of adipose tissues have seldom been performed. The purpose of our present study is conducted both in vitro and in vivo to develop a novel cryopreservation method that can be used successfully for long-term preservation of human adipose tissues for possible future clinical applica...

journal_title：Cryobiology

authors： Cui XD,Gao DY,Fink BF,Vasconez HC,Pu LL

更新日期：2007-12-01 00:00:00

abstract：:The susceptibility of in vitro matured (IVM) porcine oocytes to be fertilized in vitro after vitrification was investigated. IVM oocytes were cryopreserved by solid surface vitrification (SSV) or treated with cryoprotectants (toxicity control, TC). Control oocytes were not treated or vitrified. Live oocytes in the thr...

journal_title：Cryobiology

authors： Somfai T,Ozawa M,Noguchi J,Kaneko H,Kuriani Karja NW,Farhudin M,Dinnyés A,Nagai T,Kikuchi K

更新日期：2007-10-01 00:00:00

abstract：:The formation of ice crystals within cells (IIF) is lethal. The classical approach to avoiding it is to cool cells slowly enough so that nearly all their supercooled freezable water leaves the cell osmotically before they have cooled to a temperature that permits IIF. An alternative approach is to cool the cell rapidl...

journal_title：Cryobiology

authors： Mazur P,Pinn IL,Kleinhans FW

更新日期：2007-10-01 00:00:00

abstract：:For obligate plant-parasitic nematodes, cryopreservation has advantages over the usual preservation methods on whole plants or axenic culture systems, because the latter two are labourious and time and space consuming. In addition, cross contamination among different isolates can occur easily. Moreover, specific genet...

journal_title：Cryobiology

authors： Elsen A,Vallterra SF,Van Wauwe T,Thuy TT,Swennen R,De Waele D,Panis B

更新日期：2007-10-01 00:00:00

abstract：:Trehalose, a non-reducing glucose disaccharide found at high concentrations in many species of anhydrobiotic organisms, shows significant promise in protecting cellular viability and structural integrity during freezing and desiccation. As mammalian cell membranes are impermeable to trehalose, extensive efforts have b...

journal_title：Cryobiology

authors： Holovati JL,Acker JP

更新日期：2007-10-01 00:00:00

abstract：:Storing spores is a promising method to conserve genetic diversity of ferns ex situ. Inappropriate water contents or damaging effects of triacylglycerol (TAG) crystallization may cause initial damage and deterioration with time in spores placed at -15 degrees C or liquid nitrogen temperatures. We used differential sca...

journal_title：Cryobiology

authors： Ballesteros D,Walters C

更新日期：2007-08-01 00:00:00

abstract：:Recently, a non-invasive delipation (lipid removal) method combined with ultrarapid vitrification has been used successfully for in vitro produced (IVP) porcine embryos. In the present study, this method was combined with parthenogenesis and a recent form of somatic cell nuclear transfer (SCNT) - handmade cloning (HMC...

journal_title：Cryobiology

authors： Du Y,Zhang Y,Li J,Kragh PM,Kuwayama M,Ieda S,Zhang X,Schmidt M,Bøgh IB,Purup S,Pedersen AM,Villemoes K,Yang H,Bolund L,Vajta G

更新日期：2007-04-01 00:00:00

abstract：:Effective techniques for the cryopreservation of porcine preadipocytes could increase the usefulness of these cells as a model in obesity studies. The objective of this study was to test the effects of the following cryoprotective agents (CPAs) on the cytotoxicity, post-thaw survival, proliferation and differentiation...

journal_title：Cryobiology

authors： Li Y,Lu RH,Luo GF,Pang WJ,Yang GS

更新日期：2006-10-01 00:00:00

abstract：:We tested a "standard" cryopreservation protocol (slow cooling with 10% DMSO) on the human embryonic stem cell (hESC) line H9 containing an Oct-4 (POU5F1) promoter-driven, enhanced green fluorescent protein (EGFP) reporter to monitor maintenance of pluripotency. Cells were cooled to -80 degrees C in cryovials and then...

journal_title：Cryobiology

authors： Katkov II,Kim MS,Bajpai R,Altman YS,Mercola M,Loring JF,Terskikh AV,Snyder EY,Levine F

更新日期：2006-10-01 00:00:00

abstract：:The main aim of the present study was to investigate the effect of sperm freezing medium dilution ratio (1:1, 1:2, and 1:5 v/v), two cryoprotectants: dimethyl sulphoxide (Me(2)SO) and methanol (MeOH), and the addition of foetal bovine serum (FBS) on the cryopreservation of European eel sperm. The effect of these facto...

journal_title：Cryobiology

authors： Marco-Jiménez F,Garzón DL,Peñaranda DS,Pérez L,Viudes-de-Castro MP,Vicente JS,Jover M,Asturiano JF

更新日期：2006-08-01 00:00:00

abstract：:The development of a suitable semen extender is required to extend artificial breeding programs and to preserve the genetic potential of Bactrian camel. Experiments were conducted to provide the optimal osmolality and pH of tris-based extender and to compare that with available extenders for short-term preservation of...

journal_title：Cryobiology

authors： Niasari-Naslaji A,Mosaferi S,Bahmani N,Gharahdaghi AA,Abarghani A,Ghanbari A,Gerami A

更新日期：2006-08-01 00:00:00

abstract：:There is increasing interest in the possibility of treating diseased or damaged areas of synovial joint surfaces by grafts of healthy allogeneic cartilage. Such grafts could be obtained from cadaver tissue donors or in the future they might be manufactured by 'tissue engineering' methods. Cartilage is an avascular tis...

journal_title：Cryobiology

authors： Pegg DE,Wusteman MC,Wang L

更新日期：2006-06-01 00:00:00

abstract：:The ability to routinely cryopreserve micro-algal species reduces costs associated with maintaining large culture collections and reduces the risks of losing particular strains or species through contamination and genetic drift. Cryopreservation is also a useful adjunct in aquaculture hatcheries for strains of micro-a...

journal_title：Cryobiology

authors： Rhodes L,Smith J,Tervit R,Roberts R,Adamson J,Adams S,Decker M

更新日期：2006-02-01 00:00:00

abstract：:We investigated cryopreservation of horizontal transmission stages of Holospora undulata, a micronucleus-specific bacterial parasite of Paramecium caudatum. Unlike in previous studies on related Holospora species, protocols using glycerol as cryoprotectant failed entirely. In contrast, freezing with dimethyl sulfoxide...

journal_title：Cryobiology

authors： Millot L,Kaltz O

更新日期：2006-02-01 00:00:00

abstract：:The tetra-anionic form of ATP (ATP4-) is known to induce monovalent and divalent ion fluxes in cells that express purinergic P2X7 receptors and with sustained application of ATP it has been shown that dyes as large as 831 Da can permeate the cell membrane. The current study explores the kinetics of loading alpha,alpha...

journal_title：Cryobiology

authors： Elliott GD,Liu XH,Cusick JL,Menze M,Vincent J,Witt T,Hand S,Toner M

更新日期：2006-02-01 00:00:00

abstract：:The effects of the presence or absence of cumulus cells and equilibration times (1 and 4 min) with cryoprotectant, and Taxol treatment before vitrification (0.5-5.0 microM) on development of in vitro matured porcine oocytes after vitrification were examined. Ethylene glycol (30%) and sucrose (0.5M) was used as a vitri...

journal_title：Cryobiology

authors： Fujihira T,Nagai H,Fukui Y

更新日期：2005-12-01 00:00:00

abstract：:Antifreeze proteins are characterised by their ability to prevent ice from growing upon cooling below the bulk melting point. This displacement of the freezing temperature of ice is limited and at a sufficiently low temperature a rapid ice growth takes place. The separation of the melting and freezing temperature is u...

journal_title：Cryobiology

authors： Kristiansen E,Zachariassen KE

更新日期：2005-12-01 00:00:00

abstract：:To examine the relationship between cooling rate and cold hardiness in eggs of the migratory locust, Locusta migratoria, the survival rates and cryoprotectant levels of three embryonic developmental stages were measured at different cooling rates (from 0.05 to 0.8 degrees C min(-1)) in acclimated and non-acclimated eg...

journal_title：Cryobiology

authors： Wang HS,Kang L

更新日期：2005-10-01 00:00:00